Abstract

Antiangiogenesis, the inhibition of blood vessel formation, is a promising approach in cancer therapy, as tumors rely on angiogenesis for growth and development. This study investigates the antiangiogenic activities of ethanolic extracts from Guyabano (Annona muricata) leaves and fruit peels using the chorioallantoic membrane (CAM) assay. Fertilized chicken eggs were treated with varying concentrations of the extracts (1000 μg/mL and 2000 μg/mL) to evaluate their effects on vascular branching. The results showed a significant reduction in the mean number of branch points among different treatment groups, indicating inhibitory effects on angiogenesis. Notably, the leaf extracts exhibited stronger antiangiogenic activity than the fruit peel extract at both tested concentrations. However, there was no significant dose-dependent response observed in both extracts. These findings suggest that Guyabano extracts contain bioactive compounds, such as flavonoids and alkaloids, which may have therapeutic applications in diseases related to excessive angiogenesis. Further research is recommended to isolate and elucidate their mechanisms of action.

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